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However, the presence of this carboxylate group is necessary for the enzyme activity of the terminal cylcases that forms various cannabinoids . Therefore, OA formation via cyclization by CsOAC is required for cyclic cannabinoid biosynthesis. Cultivars exist that can be distinguished by their morphology and chemical profiles . Hemp, or fiber type cannabis, has a low Δ⁹-tetrahydrocannabinol content and grows quite tall with minimal branching while marijuana, or drug type cannabis, is shorter, has a higher degree of branching, and a high THC content. Total RNA was isolated from striata using RNATidy reagent (AppliChem. Inc., Cheshire, CT).

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